RECOMBINANT PLASMID DNA pA3GF CODING POLYPEPTIDE OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR, AND Escherichia BACTERIA RACE coli-PRODUCER OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR POLYPEPTIDE

摘要

FIELD: gene engineering. ^ SUBSTANCE: invention can be used for production of recombinant polypeptide of human granulocyte colony-stimulating factor. Recombinant plasmid DNA is constructed in vitro. It includes synthetic gene of human granulocyte colony-stimulating factor, strong constitutive promoter A3 from the early stage of bacteriophage T7 and synthetic section - translation enhancer (TREN) of gene 10 in bacteriophage T7. This DNA in combination with high copy number of plasmid and optimisation of cultivation conditions ensures constitutive biosynthesis of target protein in transformed by this DNA race of Escherichia coli SGK25/pA3GF with high yield. ^ EFFECT: constitutive biosynthesis of target protein in cells; high yield. ^ 2 cl, 4 dwg, 7 ex