FIDELITY ENHANCED ALLELE SPECIFIC AMPLIFICATION METHOD

摘要

The invention relates to a method with which an increased fidelity in the product of an Allele Specific Amplification (ASA)-assay, can be accomplished. The method is based on "primer competition" and will aid in minimising the amount of non-specific amplification product that may occur in a normal ASA amplification, when the interrogated template contains a mutated sequence. An extra primer is incorporated in the enzymatic amplification assay, the extra primer comprising a match with the mutated sequence. The extra primer will additionally comprise a modified 3'-end that will disable extension.