| 摘要 |
A method for screening an anti-cancer material is provided to screen simply the anti-cancer material based on the finding of a human breast tumor stem cell not-expressing connexin 43, expressing Oct-4, an estrogen receptor and Cripto-1 and increasing the expression of estrogen, thereby screening a chemo-preventive agent and a therapeutic agent very rapidly at low cost without using a rodent model or an in vitro model and being usefully used for studying mechanism of a human breast cancer carcinogenesis. A method for screening an anti-cancer material comprises the steps of: (a) culturing an adult stem cell or a tumor stem cell derived from a human tissue to prepare a stem cell sphere; (b) treating the sphere with an anti-cancer candidate material; (c) identifying a stem cell marker such as Oct-4, Cripto-1, ABCG2, an estrogen receptor and connexin using an antibody or a gene coupled to the stem cell marker; and (d) selecting a material where the expression of the Oct-4, Cripto-1, ABCG2, and estrogen receptor is decreased or the expression of the connexin is increased, compared with a case of not-being treated with an anti-cancer candidate material as an anti-cancer material, wherein the human tissue derived adult stem cell is an adult stem cell derived from the human tissue selected from the group consisting of breast, bone marrow, umbilical cord blood, blood, liver, skin, gastrointestinal tract, placenta and uterus and the tumor stem cell is a tumor stem cell derived from a tumor selected from the group consisting of breast cancer, brain tumor, gastrointestinal tract cancer, lung cancer, liver cancer, uterine carcinoma, large intestine cancer and myelogenous leukemia.
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