NANOFACTORY COMPOSITIONS AND METHODS OF MAKING AND USING NANOFACTORIES

摘要

The invention provides a diverse population of pharmacophores having a plurality of non-naturally occurring different structural motifs produced from the biosynthesis of unique combinations of enzymes within one or more metabolic pathways. The plurality of non- naturally occurring different structural motifs can further include a plurality of different functionalizations or other decorative modifications. Also provided is a diverse population of nanofactories that include a diverse population of pharmacophores wherein each nanofactory within the diverse population expresses at least one different non-naturally occurring structural motifs produced from the biosynthesis of unique combinations of enzymes within one or more metabolic pathways. A method of producing an isolated pharmacophore also is provided. The method includes: (a) inserting into a vector a plurality of genes encoding enzymes from one or more metabolic pathways operationally linked to expression elements; (b) introducing the vector into a host cell; (c) culturing the host cell under conditions sufficient for expression of the encoding enzymes from the metabolic pathway, and (d) isolating a compound having a non-naturally occurring structural motif biosynthetically produced from the encoding enzymes from the metabolic pathway.