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发明名称
一种分离和纯化重组腺相关病毒载体的方法
摘要
一种用于分离和纯化重组腺相关病毒载体的方法。它是通过“氯仿处理-PEG/NaCl沉淀-氯仿抽提-透析-旋转蒸发-浓缩”6个步骤分离浓缩和纯化腺相关病毒载体。利用该方法能够得到较高纯度和滴度的重组腺相关病毒载体,而且方法简便,成本较低,病毒颗粒的活性不受影响,对人体无毒性。
申请公布号
CN101173300A
申请公布日期
2008.05.07
申请号
CN200610032512.1
申请日期
2006.11.02
申请人
孙小娟
发明人
孙小娟
分类号
C12N15/861(2006.01)
主分类号
C12N15/861(2006.01)
代理机构
代理人
主权项
1.一种快速高效分离和纯化重组腺相关病毒载体的方法。其过程是:1.氯仿处理裂解细胞,加入1/10细胞悬液体积的氯仿,37℃摇床中剧烈振摇1小时。
地址
410078湖南省长沙市湘雅路88号24号
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