发明名称 |
ASSAY WITH REDUCED BACKGROUND |
摘要 |
<p>In an assay, an analyte in a sample is contacted with a thermostable reporter adenylate kinase coupled to a binding agent specific for the analyte, wherein a complex is formed. ADP is added, and then formation of ATP is monitored. Prior to the addition of ADP, endogenous kinase and uncomplexed thermostable reporter adenylate kinase is substantially removed by washing and residual-endogenous kinase is inactivated by heating. Prior to contacting the analyte with the thermostable reporter adenylate kinase, the sample has a background activity of at least 300,000 Relative Light Units per mg protein per ml sample when measured in the presence of luciferin/luciferase by a luminometer.</p> |
申请公布号 |
EP1151087(B1) |
申请公布日期 |
2008.05.07 |
申请号 |
EP20000901771 |
申请日期 |
2000.02.03 |
申请人 |
HEALTH PROTECTION AGENCY |
发明人 |
RAVEN, NEIL, DAVID, HAMMOND;WICTOME, MATTHEW, PATRICK |
分类号 |
C12N9/12;G01N33/543;C12Q1/34;C12Q1/48;C12Q1/66;G01N33/535;G01N33/58 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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