<p>The invention relates to detection the presence of a target molecule in a sample, wherein the sample is contacted with a substrate, the substrate subsequently being washed in a wash step. In particular, the invention relates to a method of detecting the presence of a target molecule in a sample, the method comprising: (a) contacting the sample (37) with a substrate having immobilized thereon probe molecules that specifically binds to the target molecule; (b) washing the substrate (38) in a wash step by a wash fluid in order to remove or dilute unbound target molecules; (c) detect the presence of resultant binding complexes (39) on the substrate to determine whether the target molecule is present in the sample- The wash fluid being substantially refractive index matched to the substrate.</p>
申请公布号
WO2008050274(A1)
申请公布日期
2008.05.02
申请号
WO2007IB54266
申请日期
2007.10.19
申请人
KONINKLIJKE PHILIPS ELECTRONICS N.V.;VAN HERPEN, MAARTEN, M., J., W.;KLUNDER, DERK, J., W.;STAPERT, HENDRIK, R.
发明人
VAN HERPEN, MAARTEN, M., J., W.;KLUNDER, DERK, J., W.;STAPERT, HENDRIK, R.