MICROORGANISM STRAIN BACILLUS SIMPLEX AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE BlsI

摘要

FIELD: biotechnology, genetic engineering, biochemistry. ^ SUBSTANCE: invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA. ^ EFFECT: valuable biological and biochemical properties of strain and endonuclease. ^ 4 dwg, 4 ex