摘要 |
An insecticidal composition for Scuticociliates is provided to improve the Scuticociliates insecticidal effect by applying an extract of pine gnarl and treat infected fish with a simple bathing method. An insecticidal composition for Scuticociliates comprises an extract of pine gnarl. The extract is prepared by crushing pine gnarl, putting 2-4 liters of methanol in 1kg of the pine gnarl crushed material, extracting it at a temperature of 20-25 deg.C 2 times, filtering and drying the extract to prepare a methanol extract, mixing the methanol extract with water and hexane in a volumetric ratio of 1:20-50:20-50, separating a water layer using separatory funnel therefrom, obtaining the remaining hexane layer and then drying the hexane layer. |