| 摘要 |
A method for reducing optical crosstalk in an optical array detector is provided. In various embodiments, the method can include measuring optical emission within a first region of interest (ROI) using a first plurality of pixels of the optical array detector, wherein each of the first plurality of pixels provides a value for optical signal intensity within the first ROI. An ROI sum signal can then be calculated by summing the values for optical signal intensity measured by the first plurality of pixels. An optical emission within a second ROI can be measured using a second plurality of pixels of the optical array detector, wherein each of the second plurality of pixels provides a value for optical signal intensity within the second ROI. The values for optical signal intensity provided by the second plurality of pixels can be algebraically manipulated to determine an optical crosstalk signal. A corrected ROI optical signal intensity can then be determined by multiplying the optical crosstalk signal by a number of pixels of the first plurality of pixels and subtracting the multiplied optical crosstalk signal from the ROI sum signal. |
