发明名称 |
Method for the manufacture of recombinant trypsin |
摘要 |
The present invention relates to a method for the manufacture and purification of recombinant trypsinogen and trypsin in E. coli and yeast, using high yield expression vectors with and without secretion leader sequences. The invention further relates to an improved method and apparatus for carrying out protein refolding specifically useful for processing trypsinogen that has accumulated intracellularly in the form of inclusion bodies.
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申请公布号 |
US7351549(B2) |
申请公布日期 |
2008.04.01 |
申请号 |
US20020181808 |
申请日期 |
2002.12.16 |
申请人 |
POLYMUN SCIENTIFIC IMMUNBIOLOGISCHE FORSCHUNG GMBH |
发明人 |
MATTANOVICH DIETHARD;KATINGER HERMANN;HOHENBLUM HUBERTUS;NASCHBERGER STEFAN;WEIK ROBERT |
分类号 |
C12N15/00;C12N9/76;C12N15/63;C12P21/04;C12Q1/68 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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