| 摘要 |
PROBLEM TO BE SOLVED: To detect ultratrace substance with high sensitivity, without having to carry out amplification of the ultratrace substance. SOLUTION: A nucleic acid strand P for detection comprises at least: a fluorescent substance F which can serve as a donor of fluorescence resonance energy; a quencher substance Q which is located at a position enabling it to receive the fluorescence resonance energy; and a nucleic acid strand part N which is located between the quencher substance Q and the fluorescent substance F, and has an enzyme cleavage site X which is cleavaged by an endonuclease. A detection technique that uses the nucleic acid strand P for the detection is also provided. COPYRIGHT: (C)2008,JPO&INPIT
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