METHOD FOR FUSION OF VEGETABLE CELL PROTOPLASTS

摘要

FIELD: biology, cellular engineering. ^ SUBSTANCE: method involves addition of lanthanum chloride to barley cell protoplasts to its final concentration 5-10 mM, and after aggregation protoplasts are incubated at 42°C for 30 min. Amount of fused protoplasts is carried out using light microscope. Protoplast fusion frequency is estimated to be 30-50%. Invention can be used for preparing large amount of barley cell fused protoplasts. ^ EFFECT: improved method for fusion of protoplasts.