METHODS FOR PURIFYING AUTHENTIC IGF FROM YEAST HOSTS

摘要

PROBLEM TO BE SOLVED: To provide a method for purifying an authentic IGF (insulin-like growth factor) from yeast. SOLUTION: The method for purifying the authentic, properly folded IGF polypeptide from a yeast cell medium comprising the IGF polypeptide comprises: (a) a step for obtaining a first IGF mixture; (b) a step for denaturing and renaturing IGF species present in the first IGF mixture to yield a second IGF mixture; (c) a step for yielding a third IGF mixture by using hydrophobic interaction chromatography; and (d) a step for performing reverse phase high performance liquid chromatography to yield a fourth IGF mixture. COPYRIGHT: (C)2008,JPO&INPIT