家蚕PIWI基因

摘要

一种家蚕PIWI基因，通过对家蚕基因组序列分析，同源比对，最后进行序列组装，设计引物钓取获得。物种间进化关系分析及基因表达模式分析显示蚕PIWI基因参与了家蚕生殖细胞内转座子活性的沉默。因此，此基因在提高家蚕转基因转化效率上具有极为重要的应用价值，可以在对胚胎干细胞进行转基因操作的同时，对此基因的表达进行干扰，减少细胞内转座子被沉默的比例，达到提高转基因转化效率的目的。此基因的研究及应用将加速实现以家蚕作为生物工厂，分子育种及通过基因操作改良蚕丝经济性状等愿望。

主权项

1.一种家蚕PIWI基因，其特征在于其序列如下：GCCCTGAATA TCTATACGAA ACGCAGAGAA AATTAAAATT GTTAGAGATA CCAACTTAGG 60ATGTCAGAAC CGAGAGGTAG AGGACGAGCT CGAGGACGCG CTGGTCGTGG TGGTGATGGA 120GGCGGACCAG CACCACGACG TCCAGGGGAA CAAGCAGGCC CATCACAACA ATCTATGCCA 180CCTGGTCCTA GACCTCAACC TCCATCTGGC TGGGGACCAC AATCCAGTGT TCCTCCAGTA 240AGAGCTGGTG TCCCGACACC AACAGCACAA GCGGGTCGCG CCTCACACCG TGTTACTCCC 300ACTACACATG AACATCCAGG AGATATCGAT GTGCAACAAC GCATGCAAAA ATTAGAACTG 360GGACCCCATT CATCTGGAGG TGGTGATGCA TCATCCGTAG TTGGCCGTGG CTCTCGACGA 420GGGGGTGGAA GAGTCCTTCC TGAAACTATT TCCATCTTGC GCACACGTCC AGAAGCTGTA 480ACATCTAAGA AAGGTACTTC GGGTACTCCC CTGGATCTGC TTGCCAACTA TTTTACTGTT 540GAAACAACTC CCAAATGGGG CCTGTATCAA TATCACGTCG ATATTTCTCC AGAAGAAGAC 600AGCACTGGAG TCCGAAAGGC ACTGATGCGT GTTCACAGCA AAACACTCGG AGGATATCTC 660TTTGATGGCA CTGTGTTGTA CACTGTAAAT CGTCTGCATC CTGATCCGAT GGAGCTATAT 720TCTGATCGTA AAACGGATAA CGAACGTATG CGAATCTTAA TTAAGCTGAC CTGCGAAGTT 780AGCCCTGGAG ACTATCACTA TATACAAATC TTTAACATCA TTATAAGGAA ATGCTTTAAT 840TTATTGAAGC TACAGCTTAT GGGACGGGAT TATTTTgaCC CTGAAGCAAA GATTGATATA 900CCAGAATTCA AGCTTCAAAT TTGGCCAGGA TATAAAACGA CAATCAATCA ATATGAAGAT 960CGTTTGTTGT TGGTCACTGA AATCGCCCAT AAGGTGCTTC GCATGGACAC TGTTCTTCAA 1020ATGTTGAGTG AATATGCCGC AACAAAAGGC AATAATTACA AAAAAATATT TTTAGAAGAT 1080GTTGTTGGAA AAATTGTAAT GACTGACTAC AATAAAAGAA CTTACAGAGT TGACGATGTA 1140GCTTGGAATG TGTCGCCGAA GTCCACATTT AAAATGCGTG ACGAAAACAT TACCTATATT 1200GAATACTATT ATAAGAAATA CAATCTACGC ATTCAAGATC CGGGTCAACC GCTTCTGATT 1260TCGCGGTCGA AGCCGCGTGA GATTCGTGCG GGTCTGCCTG AGTTGATATA TCTAGTGCCA 1320GAACTGTGCC GTCAGACCGG CCTCTCCGAC GAGATGCGAG CTAATTTCAA GCTGATGCGC 1380TCATTGGACG TCCATACAAA GATTGGGCCC GACAAGCGCA TTGAAAAGCT TAATAATTTC 1440AACAGGAGAT TCACAAGCAC ACCTGAAGTT GTTGAAGAAC TGGCAACATG GTCGTTGAAA 1500CTATCTAAGG AACTGGTAAA AATAAAGGGT CGCCAACTTC CACCTGAAAA TATAATACAA 1560GCAAATAATG TTAAATACCC TGCAGGAGAC ACCACCGAGG GCTGGACGCG AGATATGAGA 1620TCGAAGCATC TCCTGGCTAT TGCCCAACTA AACTCTTGGG TAGTGATCAC CCCAGAAAGA 1680CAACGCCGTG ATACAGAGTC GTTCATTGAT CTCATAATAA AAACTGGCGG AGGTGTTGGT 1740TTTCGAATGA GAAGCCCTGA CTTAGTTGTG ATTCGTCATG ACGGTCCGAT AGAATACGCT 1800AATATGTGTG AAGAAGTGAT TGCAAGGAAA AATCCTGCCC TGATACTGTG CGTTCTGGCT 1860AGGAATTATG CTGATAGGTA TGAAGCCATA AAAAAGAAAT GCACTGTAGA CCGAGCCGTG 1920CCGACGCAGG TAGTCTGCGC TCGTAACATG AGTAGCAAGT CGGCTATGTC AATTGCTACA 1980AAAGTTGCTA TTCAAATAAA CTGTAAGCTT GGGGGTTCTC CGTGGACAGT AGATATCCCA 2040CTGCCGTCAC TGATGGTGGT CGGGTACGAC GTGTGCCACG ACACTCGTTC GAAAGAAAAG 2100AGCTTCGGGG CCTTCGTAGC CACCCTCGAC AAACAGATGA CTCAGTACTA TTCAATCGTC 2160AATGCGCACA CTTCCGGAGA AGAACTCAGC TCTCACATGG GTTTCAATAT CGCGTCCGCT 2220GTCAAAAAAT TCCGCGAGAA AAACGGGACC TACCCAGCCC GAATCTTCAT TTACCGCGAC 2280GGTGTCGGTG ATGGTCAAAT CCCATACGTG CATAGCCATG AAGTTGCTGA GATAAAAAAG 2340AAATTGGCAG AAATTTATGC AGGAGTCGAA ATAAAGTTGG CATTCATTAT TGTGTCGAAA 2400CGTATTAACA CCCGAATCTT CGTTCAACGT GGTCGCAGCG GAGAAAATCC ACGCCCTGGC 2460ACAGTGATTG ACGACGTCGT CACCTTGCCT GAACGGTATG ATTTCTATCT GGTTTCACAA 2520AATGTACGTG AAGGTACCAT TGCTCCTACC TCATACAATG TGATTGAAGA TACAACAGGA 2580CTCAATCCTG ATCGTATCCA ACGTTTGACC TACAAACTGA CACACTTGTA TTTCAACTGC 2640TCTTCCCAAG TTCGCGTCCC ATCGGTCTGC CAATATGCCC ACAAACTTGC ATTCCTTGCA 2700GCTAACAGTC TGCACAACCA ACCGCATTAT TCATTGAATG AAACTTTATA TTTCCTCTAA 2760GTATTTTTAA AGACAACCTA TTTTTTTCTA GATTAAGTAA GTGTACCCAC CGAGAAACTA 2820TTTTCGATGT GCATAACCAT ACAAATCATA GAACTCTATT AGAAATAGTT CGCAATCATA 2880ACGTGAGCTT AATATTGCTC AAAGTAATTT CATTTCATAG TTTTTTAAAA GATATATTAA 2940ATAAGACTCA TTCTCTTGGA CGTTTTTGAT GTATGATTAG TGTGGCATAG CAAGAAGCAG 3000CTAATGCGCG TAGTAGTAGT ACATGTGACC TAGACTCGTT TTTACGATAA TTAAAATGTA 3060ATGAGTGTCC TAGTTGTTTA CTACATAAGA TTCATTTTAT ATTTGATTGA CAATTGTCAA 3120ATTTTTCTTT TTATTTTGGC TACAATGATG TTGAGTGGAA ATGCAAACGA ATAATTATTA 3180AATTACAAGT TATTCAAATT GAATCAAGTG CATTCTTCCA CGCTACAAGC AGTGAT 3236