METHOD FOR THE PRODUCTION OF INSULIN-LIKE GROWTH FACTOR-I

摘要

Method for the production of IGF-I, characterized by cultivating a prokar yotic host cell comprising an expression vector containing a nucleic acid en coding a fusion protein comprising said IGF-I N-terminally linked to the C-t erminus of a propeptide, whereby said propeptide ends C-terminally with amin o acids -Y-Pro, wherein Y is selected from the group consisting of Pro, Pro- Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, or Pro-Arg-Pro, r ecovering and cleaving said fusion protein with IgA protease, and recovering said IGF-I. IGF-I is useful for the treatment of neurodegenerative disorder s like Alzheimer's Disease.