A METHOD OF AMPLIFYING A NUCLEIC ACID FROM A MICROORGANSIM USING A NONPLANAR SOLID SUBSTRATE

摘要

A method for amplifying a nucleic acid from a microorganism is provided to amplify conveniently, rapidly and highly sensitively a nucleic acid by performing separation of the microorganism, cell lysis and amplification of a nucleic acid in one container and use a large amount of an initial sample due to contact the microorganism with high fluid speed with a solid substrate. A method for amplifying a nucleic acid from a microorganism such as bacteria, fungi or virus comprises the steps of: (a) contacting a non-planar solid substrate with a biological sample including the microorganism under pH of 3.0-6.0 to attach the microorganism to the solid substrate; (b) washing materials not attached to the solid substrate to remove the materials; and (c) subjecting the microorganism attached to the solid substrate to PCR, wherein all the steps are performed in a same container and the solid substrate is selected from the group consisting of a solid substrate having a pillar structure where a plurality of pillar is formed, a bead-shaped solid substrate and a sieve-structured solid substrate, has a water contact angle of 70-95° and includes at least one amine-based functional group on the surface thereof.