METHOD OF DESIGN PROBES FOR DETECTING TARGET SEQUENCE AND METHOD OF DETECTING TARGET SEQUENCE USING THE PROBES

摘要

A method for designing a probe is provided to obtain the probe which is able to detect rapidly and accurately existence of a target sequence in a reaction sample including the target sequence and many non-target sequences. And a method for detecting a target sequence is provided to be able to detect rapidly and accurately existence of the target sequence in the reaction sample. A method for designing a probe for detecting a target sequence comprises the steps of: (a) selecting a position in a target sequence different from a non-target sequence which has the highest sequence homology to the target sequence to be detected as a standard position; (b) selecting a first probe design area for all probes having a certain length designed in a certain area in the target sequence including the standard position; (c) selecting a position in the target sequence different from the non-target sequence as a probe selection position; (d) selecting a complete match probe including the probe selection position at the center position and consisting of a completely hybridizable sequence; and (e) selecting an intentional mis-match probe which is longer than the complete match probe, includes the probe selection position at the first position and a nucleotide, unable to be hybridized with the target sequence, and consists of a sequence which is completely hybridizable with the target sequence except the second position. A method for detecting a target sequence using the probe designed by the method above comprises the steps of: (a) providing a reaction sample to detect the existence of the target sequence to the complete match probe and the intentional mis-match probe; and (b) identifying the hybridization between the probes and the target sequence.