| 摘要 |
The present invention is intended to provide a diagnostic method for paratuberculosis by which an animal infected with Mycobacterium avium subsp. Paratuberculosis can be diagnosed at a high sensitivity in the sub-clinical infection stage before the specific antibody level begins to increase and a large number of specimens can be treated. The present invention provides: a diagnostic method for paratuberculosis characterized by collecting the blood of a subject animal, adding an anti-IL-10 antibody and a Mycobacterium avium subsp. Paratuberculosis antigen to the collected blood followed by culture, and then measuring the amount of produced IFNgamma in the blood; a diagnostic method for paratuberculosis characterized in that the amount of produced IFNgamma in blood is measured by the IFNgamma ELISA method; and a diagnostic method for mycobacterial infection characterized by collecting the blood of a subject animal, adding an anti-IL-10 antibody and a mycobacterial antigen to the collected blood followed by culture, and then measuring the amount of produced IFNgamma in the blood.
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