DNA POLYMERASE COMPOSITIONS FOR QUANTITATIVE PCR AND METHODS THEREOF
摘要
The invention relates to the generation and characterization of Archaeal DNA polymerase mutants with deficient 3'-5' exonuclease activity and reduced base analog detection activity. The invention further provides for Archaeal DNA polymerase mutants with deficient 3'-5' exonuclease activity and reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of DNA polymerases with efficient 3'-5' exonuclease activity and reduced base analog detection activity.
申请公布号
WO2005059159(A3)
申请公布日期
2008.01.24
申请号
WO2004US41899
申请日期
2004.12.13
申请人
STRATAGENE CALIFORNIA;SORGE, JOSEPH, A.;MUELLER, REINHOLD, DIETRICH;PADMABANDU, GOTHAMI;ROELOFS, NICK;HOGREFE, HOLLY, H.
发明人
SORGE, JOSEPH, A.;MUELLER, REINHOLD, DIETRICH;PADMABANDU, GOTHAMI;ROELOFS, NICK;HOGREFE, HOLLY, H.