Discrimination method of target base in DNA, and allele specific primer used in the method of the same

摘要

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is T. According to the present invention, the 3' end base is designed to be the base corresponding to SNP; the second base from the 3' end to be T or G; the third base from the 3' end to be G or C; and the base sequence of from the fourth from the 3' end to the 5' end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3' side to a desired base.