<p>The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3' end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.</p>
申请公布号
WO2008007951(A1)
申请公布日期
2008.01.17
申请号
WO2007NL00177
申请日期
2007.07.10
申请人
KEYGENE N.V.;VAN EIJK, MICHAEL, JOSEPHUS, THERESIA;JESSE, TACO, PETER
发明人
VAN EIJK, MICHAEL, JOSEPHUS, THERESIA;JESSE, TACO, PETER