| 摘要 |
scFv antibodies which specifically bind selected antigens and are obtaina ble by a method comprising (i) selecting from a pool of soluble and stable a ntibody frameworks a soluble and stable framework matching best the framewor k of a non-human antibody against the antigen with a certain binding specifi city, (ii) either providing said soluble and stable framework with CDRs that bind specifically to said antigen, or mutating the framework of said non-hu man antibody towards the sequence of said soluble and stable framework, to g enerate scFv antibodies, (iii) testing the generated antibody for solubility and stability, and testing the generated antibody for antigen binding, and (iv) selecting an scFV that is soluble, stable and binds to the antigen spec ifically. Also provided are pharmaceutical compositions comprising said scFv antibody, methods of treatment and diagnosis for diseases related to over e xpression of antigens that are specifically bound by said antibody. |
