发明名称 |
Selective cloning of homoduplex nucleic acids |
摘要 |
The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
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申请公布号 |
US2008014634(A1) |
申请公布日期 |
2008.01.17 |
申请号 |
US20060637395 |
申请日期 |
2006.12.11 |
申请人 |
GREENER ALAN L;HEXDALL LISA J;CARSTENS CARSTEN-PETER;SORGE JOSEPH A |
发明人 |
GREENER ALAN L.;HEXDALL LISA J.;CARSTENS CARSTEN-PETER;SORGE JOSEPH A. |
分类号 |
C12N5/10;C12N1/21;C12N9/22;C12N15/10 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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