| 摘要 |
<p>The present invention relates, e.g., to a method for screening for mutations in a DNA sample, comprising (a) diluting and distributing the sample to obtain a plurality of aliquots which, on the average, contain between about 2-10 genome equivalents of the DNA; (b) PCR amplifying the DNA in a sufficient number of aliquots, with at least one set of PCR primers, so as to generate amplicons such that, if mutations are present at a frequency of about 1-10% in a given gene in the sample, the probability of there being a mutation in that gene in at least one of the amplicons is at least about 95%; and (c) screening the amplicons for the presence of mutations, using a method that can detect unspecified mutations at unspecified sites within an amplicon (e.g., temperature gradient capillary electrophoresis (TGCE) or cycle sequencing of the DNA).</p> |
