METHOD FOR PREPARING OF MESENCHYMAL STEM CELL BY ULTRASOUND TREATMENT

摘要

A method for preparing mesenchymal stem cells is provided to improve the adhesion capability of the mesenchymal stem cells by treating cells including the mesenchymal stem cells with ultrasonic waves, thereby easily mass-obtaining the pure mesenchymal stem cells having the normal differentiation capability from the cell including the mesenchymal stem cells. A method for preparing mesenchymal stem cells from a cell group containing the mesenchymal stem cells comprises the steps of: (a) culturing the cell group containing the mesenchymal stem cells in a culture medium with treating the cell or the culture medium with 5-50 minutes/day of supersonic waves having the intensity of 10-1,000 mW/cm^2 for 1-12 days; and (b) removing the cell not attached to the culture medium and obtaining colony(CFU-Fs) forming mesenchymal stem cells attached to the culture medium.