发明名称 |
Frontal affinity chromatography/MALDI tandem mass spectrometry |
摘要 |
Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.
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申请公布号 |
US7317187(B2) |
申请公布日期 |
2008.01.08 |
申请号 |
US20050135490 |
申请日期 |
2005.05.24 |
申请人 |
DAVIDSON WILLIAM R;SHUSHAN BORI;KOVARIK PETER;COVEY TOM R;BRENNAN JOHN D |
发明人 |
DAVIDSON WILLIAM R.;SHUSHAN BORI;KOVARIK PETER;COVEY TOM R.;BRENNAN JOHN D. |
分类号 |
B01D59/44;H01J49/04;H01J49/16 |
主分类号 |
B01D59/44 |
代理机构 |
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代理人 |
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地址 |
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