Pathogen-inducible synthetic promoter

摘要

#CMT# #/CMT# Pathogen-inducible synthetic promoter for regulating the transcription of a nucleic acid comprises a minimal prometer with a defined sequence motif located downstream of a TATA region and upstream of a transcription startpoint. #CMT# : #/CMT# Pathogen-inducible synthetic promoter for regulating the transcription of a nucleic acid comprises a minimal prometer with a DBRMWA or TWCCCMT sequence motif located downstream of a TATA region and upstream of a transcription startpoint. Independent claims are also included for: (1) recombinant gene with a promoter as above; (2) plant cell in which a promoter as above is integrated into the cell's DNA; (3) seed material of a transgenic plant as above. #CMT#USE : #/CMT# The promoter is useful for producing transgenic plants with broad resistance to pathogens, e.g. fungi, oomycetes, bacteria, viruses, insects and nematodes. #CMT#ADVANTAGE : #/CMT# The promoter has low background activity and a high induction rate. #CMT#DESCRIPTION OF DRAWINGS : #/CMT# The drawing shows a sequence comparison for seven minimal promoters as above (SEQ ID NO:1-7). #CMT#BIOTECHNOLOGY : #/CMT# Preferred Promoter: The sequence motif is present two or more times. The minimal promoter has SEQ ID NO:1-9 (defined sequences given in the specification). The promoter also includes a cis element with SEQ ID NO:10-12 (defined sequences given in the specification). #CMT#EXAMPLE : #/CMT# Potato plants having a luciferase reporter gene under the control of the minimal promoter of the StPSBR gene (SEQ ID NO:7) exhibited only 18% of the mean background activity of the 35S minimal promoter, while 72 hours after infection with Phytophthora infestans the induction factor was 395, compared with 10 for the 35S minimal promoter.