| 摘要 |
A method of sample displacement chromatography separation which comprises applying a multicomponent sample to one end of a chromatography bed comprising stationary phase material having affinity for components of the sample, causing components of the sample to become distributed along the chromatography bed by passage over the bed o f a non-eluting mobile solvent phase, and recovering a desired component of the sample from at least a portion of the chromatograph y bed, in which the sample components are applied to the chromatography bed in a non-homogeneous manner such that the concentration o f at least one component with relatively low affinity for the stationary phase material is enhanced during an earlier part of sample application and/or the concentration of at least one component with relatively high affinity for the stationary phase material is enhanced during a later part of sample application.
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