PREPARATION METHOD FOR THE PRODUCTION OF ACTIVE PROTEIN COMPLEX PARTICLES USING CELLULOSE BINDING DOMAIN

摘要

A method for production of active protein complex particles by using a cellulose binding domain is provided to produce the active protein complex particles easily and rapidly by expressing in Escherichia coli without pretreatment or immobilization, recover and recycle the particles by centrifugation, and increase immobilization rate by treating with a crosslinking agent. A method for production of active protein complex particles comprises the steps of: preparing a recombinant vector containing a gene encoding cellulose binding domain derived from Cellulomonas fimi having the amino acid sequence of SEQ ID NO:1 and an active protein gene; transforming a host cell with the recombinant vector; and culturing the transformed microorganism, wherein the cellulose binding domain is exoglucanase; the active protein is beta-glucuronidase(gusA) or beta-glucosidase(bglA); the host cell is Escherichia coli; and the active protein complex particles are immobilized by adding a crosslinking agent such as genipin or glutaraldehyde into the particles.