METHOD FOR ISOLATING VIRUS

摘要

It is intended to provide a novel isolation method capable of isolating a virus with an excellent efficiency, and particularly, a method capable of isolating a noninfectious virus as well. A nucleic acid fraction or a virus fraction in a sample is extracted and introduced into a target host cell by a method of introducing a nucleic acid into a cell noninfectiously and directly. By introducing the nucleic acid fraction or the like into the target host cell directly like this, the virus can be replicated in the host cell without the influence of virus infectivity to the host cell and a mature virus can be isolated. As the introduction method, for example, a calcium phosphate method, a polyethyleneglycol method, a lipofection method, an electroporation method, an ultrasound transfection method, an introduction method utilizing a gene gun or the like can be used.