POLYNUCLEOTIDES COMPRISING SINGLE NUCLEOTIDE POLYMORPHISM, MICROARRAYS AND DIAGNOSTIC KITS COMPRISING THE SAME, AND METHODS FOR DIAGNOSING PREMATURE OVARIAN FAILURE USING THE SAME

摘要

Polynucleotides comprising SNP(single nucleotide polymorphisms) associated with premature ovarian failure, present in BCKDHB(branched chain keto acid dehydrogenase, E1-beta subunit) gene are provided to diagnose the premature ovarian failure of female, thereby being usefully used as marker for clinical diagnosis of premature ovarian failure. A polynucleotide comprises 10 or more consecutive DNA sequences containing (a) 201st base of C(cytosine), (b) 201st base of C, (c) 131st base of T(thymine), (d) 201st base of A(adenine), (e) 201st base of T, (f) 805th base of G(guanine), (g) 201st base of T, (h) 201st base of A, (i) 201st base of C or (j) 501st base of A as a single nucleotide polymorphism in the nucleotide sequence of SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 9 or 10. A method for diagnosis of premature ovarian failure comprises the steps of: obtaining a nucleic acid sample from a subject; hybridizing the sample with a microarray having the polynucleotide or its complementary polynucleotide; and detecting the hybridization results.