摘要 |
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds. |
申请人 |
RAINDANCE TECHNOLOGIES, INC.;LINK, DARREN, R.;BOITARD, LAURENT;BRANCIFORTE, JEFFREY;CHARLES, YVES;FEKE, GILBERT;LU, JOHN, Q.;MARRAN, DAVID;TABATABAI, AHMADALI;WEINER, MICHAEL;HINZ, WOLFGANG;ROTHBERG, JONATHAN, M. |
发明人 |
LINK, DARREN, R.;BOITARD, LAURENT;BRANCIFORTE, JEFFREY;CHARLES, YVES;FEKE, GILBERT;LU, JOHN, Q.;MARRAN, DAVID;TABATABAI, AHMADALI;WEINER, MICHAEL;HINZ, WOLFGANG;ROTHBERG, JONATHAN, M. |