发明名称 |
Using a reverse genetic engineering platform to produce protein vaccines and protein vaccine of avian influenza virus |
摘要 |
The present invention relates to a preparation method of protein vaccines, and comprises the steps of: (a) providing at least one amino acid sequence of an epitope of a target antigen protein; (b) converting the amino acid sequence into a nucleic acid sequence and modifying the codons; (c) synthesising a plurality of primers of the modified nucleic acid sequence; (d) synthesising the modified nucleic acid sequence in vitro; (e) inserting the synthesized fragment of the modified nucleic acid sequence into a plasmid; (f) transforming the plasmid into a host cell to produce the modified nucleic acid encoded epitope peptide; and (g) collecting and purifying the produced peptide.
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申请公布号 |
US2007243587(A1) |
申请公布日期 |
2007.10.18 |
申请号 |
US20070783300 |
申请日期 |
2007.04.09 |
申请人 |
HEALTHBANKS BIOTECH CO., LTD. |
发明人 |
LIAO CHAO-WE;CHANG HSIU-KANG;HWANG KINKAI |
分类号 |
C12P21/06;C07H21/04;C07K14/08;C12N7/02;C12N15/86 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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