| 摘要 |
Disclosed is a method and apparatus for mea-suring an analyte in a tissue of a subject. The method comprises contacting the tissue with elec-tromagnetic radiation having a first excitation wave-length, wherein the first excitation wavelength is substantially equal to an absorption wavelength of a temperature probe within the tissue. The tempera-ture probe and the analyte are sufficiently proximate to one another that energy deposited into one by ab-sorption of radiation is transferred to the other. The Raman spectra emitted by the tissue are collected and analyzed to determine a concentration of ana-lyte present in the tissue. The analysis can com-prise measuring the Raman spectra associated with the temperature probe. In addition, the method can include simultaneously contacting the tissue with electromagnetic radiation having the first excitation wavelength and with electromagnetic radiation hav-ing a second excitation wavelength, wherein the second excitation wavelength is substantially equal to an absorption wavelength of the analyte. The analysis comprises comparing the spectra emitted in response to the first excitation wavelength in the presence and in the absence of the second excita-tion wavelength. In another embodiment, the analy-sis comprises measuring the anti-Stokes component of the Raman spectra associated with the analyte. The method provides a non-invasive measurement of blood glucose, using hemoglobin as the temper-ature probe.
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