发明名称 |
METHODS AND COMPOSITIONS FOR THE PREPARATION AND USE OF FIXED-TREATED CELL-LINES AND TISSUE IN FLUORESCENCE IN SITU HYBRIDIZATION |
摘要 |
This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by "mRNA liberation in fixed-treated tissue or 'MLIFTT'". This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence and the physical treatments overcome the interference created by the fixation-induced chemical bonds. The methods of the present invention can be utilized to identify a plurality of mRNA transcripts in a microarray format. |
申请公布号 |
EP1689883(A4) |
申请公布日期 |
2007.10.10 |
申请号 |
EP20040778619 |
申请日期 |
2004.07.19 |
申请人 |
AUREON BIOSCIENCES CORPORATION |
发明人 |
EDELSON, JON;CAPODIECI, PAOLA |
分类号 |
C12Q1/68;C07H21/04;G01N33/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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