BIOSENSOR HAVING NANO WIRE FOR DETECTING FOOD ADDITIVE MONO SODIUM GLUTAMATE AND MANUFACTURING METHOD THEREOF

摘要

A biosensor is provided to prevent the degradation of electrical characteristics of nano wire and sensitively detect glutamate even through a small amount thereof is contained in a food, thereby being able to effectively be used in detecting food additives existing in processed foods. A biosensor for detecting glutamate comprises a solid substrate; at least one signal transducer which is arranged in a matrix and has nano wires of which both ends are adhered to electrodes; and at least one signal sensing part which is disposed between the nano wires on a surface of the solid substrate and to which glutamate oxidase to be bound to glutamate is adhered, wherein the glutamate is monosodium glutamate and L-glutamate, the solid substrate is a silicon substrate or a glass substrate and the nano wire is at least one selected from the group consisting of a carbon nanotube, a silicon nano line, a zinc oxide nano wire and a vanadium oxide nano wire. A method for preparing a biosensor for detecting glutamate comprises the steps of: (a) integrating nano wires on a surface of a solid substrate; (b) coating electrodes with a polymer after forming the electrodes at both ends of each of the nano wires; (c) adhering functional groups between the nano wires which are on the surface of the solid substrate; (d) adhering glutaraldehyde to the functional groups on the surface of the solid substrate; and (e) immobilizing glutamate oxidase which is able to be bound to monosodium glutamate and catalyze the oxidative reaction of glutamate to glutaraldehyde.