发明名称 |
R-HYDROXYNITRILE LYASES HAVING IMPROVED SUBSTRATE ACCEPTANCE AND THE USE THEREOF |
摘要 |
R-hydroxynitrile lyases having an improved substrate acceptance, increased activity and increased selectivity, in which there is replacement in the amino acid sequence of R-hydroxynitrile lyases from the Rosaceae family either a) of the amino acid residue which corresponds to position 360 of the mature PaHNL5 protein by another apolar amino acid or a neutral amino acid and/or b) of the amino acid residue which corresponds to position 225 of the mature PaHNL5 protein by another polar amino acid, it also being possible where appropriate for 1 to 20 further residues in the active center or in the hydrophobic channel leading to the active center to be replaced. |
申请公布号 |
EP1838855(A1) |
申请公布日期 |
2007.10.03 |
申请号 |
EP20050825158 |
申请日期 |
2005.12.28 |
申请人 |
DSM FINE CHEMICALS AUSTRIA NFG GMBH & CO KG |
发明人 |
SKRANC, WOLFGANG;GLIEDER, ANTON;GRUBER, KARL;WEIS, ROLAND;MAURER, OLIVER;GAISBERGER, RICHARD |
分类号 |
C12N15/52;C07K14/415;C12N9/88;C12P13/00 |
主分类号 |
C12N15/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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