Fast method of transforming competent cells

摘要

The invention relates to a fast method of transforming competent cells, comprising: a. mixing a plasmid DNA with the competent cells suspending within ionic solution to form a mixture; b. plating the mixture on a warm selective medium; and c. culturing the mixture on the medium; wherein the ionic solution comprises divalent cation selected from the group consisting of Ca²⁺, Mg²⁺, Mn²⁺, Zn²⁺, Cu²⁺, Cd²⁺, Fe²⁺, Sr²⁺ and Co²⁺, and provided that the ionic solution does not include Ca²⁺ alone.