Sample structure imaging method, involves repeatedly exciting fluorescence dye with excitation light, and stabilizing ratio of yield at fluorescence light to concentration of dye to value

摘要

The method involves repeatedly exciting a fluorescence dye within an area with an excitation light for an emission of a fluorescence light. The fluorescence light is registered in an area of the dye to determine a spatial concentration distribution of the dye. The dye is supplied with an optical signal of wavelength for the excitation light in the area of the dye before the repeated excitation. A ratio of yield at the fluorescence light to the concentration of the dye is stabilized to a value (21). An independent claim is also included for a device for imaging a structure, comprising a cooling device.