发明名称 |
Protein and process for preparing the same |
摘要 |
A protein having amino acid sequence in SEQ ID NO: 1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID NO: 1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
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申请公布号 |
US7268114(B2) |
申请公布日期 |
2007.09.11 |
申请号 |
US20030365231 |
申请日期 |
2003.02.12 |
申请人 |
BIOPHARM GESELLSCHAFT ZUR BIOTECHNOLOGISCHEN ENTWICKLUNG PHARMAKA MBH |
发明人 |
MAKISHIMA FUSAO;TAKAMATSU HIROYUKI;MIKI HIDEO;KAWAI SHINJI;KIMURA MICHIO;MATSUMOTO TOMOAKI;KATSUURA MIEKO;ENOMOTO KOICHI;SATOH YUSUKE |
分类号 |
A61K38/18;A61F2/28;A61F2/30;A61K38/00;C07K14/51;C12N1/21;C12N15/12;C12P21/02 |
主分类号 |
A61K38/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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