| 摘要 |
In one aspect, the present invention is directed to a process for isolating proteins from a solution comprising proteins, wherein the solution is selected from the group consisting of: crude bood plasma, blood serum, cryosupernatant derived from plasma, fractionated human plasma, cryoprecipitate derived from plasma and recombinant broths. The process involves providing a solid separation medium having the formula: M-S-L wherein M is a matrix backbone, S is an optional spacer arm, and L is a ligand which is mercaptonicotinic acid, contacting the solid separation medium with the solution comprising the proteins, such that at least one of the proteins becomes reversibly bound bound to said solid separation medium. At least one elution step is then performed to selectively elute at least one protein fraction from the solid separation medium. In another aspect, the present invention is directed to a process for isolating Factor VIII and/or Factor IX.
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