METHOD FOR SYNTHESIS OF SUPPRESSOR tRNA, DNA CONSTRUCT, AND PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE DNA CONSTRUCT

摘要

Disclosed is a DNA construct comprising: a non-eukaryotic suppressor tRNA gene which contains no internal promoter sequence capable of functioning in an eukaryotic cell; and an eukaryotic or bacteriophage-derived promoter ligated to the 5'-terminus of the tRNA gene. Also disclosed is a method for synthesis of suppressor tRNA using the DNA construct. Further disclosed is a method for production of a protein having a non-natural amino acid integrated therein by using the DNA construct.