METHOD FOR DETECTION OF NEWCASTLE DISEASE RNA-VIRUS

摘要

A method for detection of Newcastle disease RNA-virus comprises RNA extraction, its reverse transcription and amplification of kDNA-virus of Newcastledisease in gene F area and primers "NDVfusionFF" (5' AGG CCT CTT GCA GCT GCA G 3'), "NDVfusionRR" (5' GTT GCA ACC CCA AGA GCT ACA C 3') and "lasotafusionR" (5' GTT GCA ACC CCA AGA GCC ACA CC 3') are used at annealing temperature of 60 and 62 DEGREE C and synthesis of a motiff of length of 345 p.n.