| 摘要 |
FIELD: biotechnology, in particular production of glycopeptide-originated biologically active substances. ^ SUBSTANCE: claimed method includes cultivation of lactic acid bacteria on nutrient medium containing defatted desiccated milk and yeast extract, followed by treatment of cultural liquid with acidic proteolytic enzymes, hydrolysis, separation of cultural liquid by centrifugation on biomass and milky whey which are treated separately. Milky whey is separated by ultrafiltration on high- and low molecular fractions. High molecular fraction of milky whey is lyophilized. Low molecular fraction of milky whey is vaporized to produce lactic also is disclosed or salts thereof. Biomass is suspended in ammonia water and separated by centrifugation on liquid and solid phases. Liquid phase is concentrated by ultrafiltration. Zinc acetate solution is added to obtained concentrated to produce zinc-containing complex. Solid phase (biomass) is treated with ultrasound or by "freezing/defrosting" method for cell wall decomposition followed by boiling, cooling, lysocim addition, and hydrolysis. Hydrolyzate is acidified with acetic acid and separated on liquid and solid phases. Solid phase obtained from hydrolyzate is dried to produce preparation containing cell wall residues and non-dissolved glycopeptide. Liquid phase obtained from hydrolyzate is fractioned by ultrafiltration on high- and low molecular fractions. High molecular fraction is lyophilized to produce preparation containing lysocim and high molecular glycopeptide. Low molecular fraction is treated by chromatography, filtered, boiled down, and lyophilized to produce preparation. ^ EFFECT: improved method for glycopeptide production. ^ 3 cl, 1 tbl, 3 ex |
