MANIPULATION OF PHOTOSYNTHESIS AND STARCH CONTENT OF HIGHER PLANTS BY A CYANOBACTERIAL GLYCOGEN SYNTHASE

摘要

A method for preparing a transformed plant is provided to obtain the transformed plant which is able to express glycogen synthetase and significantly increase the production amount and the size of tuber during in vitro culture, decreases the starch particle size of a potato tuber and has constant size distribution, and be able to increase the potato production amount per area in accordance with the starch concentration increase. The DNA coding glycogen syntase cglgA derived from Synechocystis sp. PCC6803 includes a sequence described as SEQ ID : NO. 1. The method for preparing a transformed plant expressing the cglgA comprises the steps of: (a) transforming plant cells using a polynucleotide sequence having (i) a nucleotide sequence coding the cglgA and described as SEQ ID : NO. 1, (ii) a promoter which is operably linked to the nucleotide sequence(i) and acts on the plant cells to form RNA molecules, and (iii) 3'-non-translation portion which acts on the plant cells to induce polyadenylation of 3'-terminal of the RNA molecules; (b) selecting the transformed plant cells using an Agrobacterium tumefaciens-binary vector system; and (c) after obtaining a redifferentiated shoot from the transformed plant cells, culturing the shoot in a culture medium to obtain a transformed plant.