苎麻微卫星DNA标记

摘要

本发明涉及一种苎麻的DNA分子遗传标记，提供了15个苎麻微卫星位点的DNA序列及扩增上述15个苎麻微卫星位点的引物序列和扩增方法，15个苎麻微卫星位点用于苎麻品种分类、遗传关系分析和标记辅助育种，重复性好，是一种可靠有效的分子标记。

主权项

1.一种苎麻微卫星标记，其特征在于，所述微卫星标记的编号分别为BN19、BN20、BN21、BN22、BN23、BN24、BN25、BN26、BN27、BN28、BN29、BN30、BN31、BN32、BN33，其核苷酸序列是：BN19：taaattgtga aggccatttt gggccttcac tttgttgaat gcccaaccca tttatgggcc 60taacagcaag aaagctgtga aggctatttt cgaccttgtt ctagcgcctc ataagtggct 120aacgcccatt tgtatcttgg ccaaatgtgt aagaaggcct tgaacctagt agtgggccac 180ttaattactc aaagagagag agagagagag agagagagag agagagagag agagagagag 240agagagagag agagagagag agagagagag aggggttttc tgagatgggc tagctcaaag 300tcaggtgggc ctggcgttgg cccgaaatga ccaaactcga cccgacccgg aagtgaccaa 360cctcgggtca agaagtgaca cgtggacaga agtacctaac tgtcagcctc aggtaatttc 420atggaagtgg ataaagaaac agagagagag agagagcgcg agattttagc gccttttaac 480cagacacaac cgttccaagt tttgaaaagc gctataaggg tgaatctgct ccacgacaga 540ttcgatttca tcattacttt cgaacccaaa agacttcaat tgacgaaagc cttctttatc 600taattgataa acaatatttt tgacacccgc aaatccatat aagtgcaata cttcagattt 660tttcacgatc attt 674BN20：taaggcttgt tggacctcca agtgagtccc taatacttat tataatatat tatatgttca 60aatactcagt tgggatcaaa acaactttgt tagagtcgct tcggagccaa aaaactattt 120tgaaggctat atttcgacaa aactagttat aacgatttac tttttgcgcg aaataaattt 180ttaagcttgg agtagtcaag agtggttgaa gctctaacca ctagagattt tgagaagcgc 240acagtatgag cgaaaatggg cttgtgggtt ttggtgtaga tggcaaagga aggtgaagaa 300caagcgtaga atgtcgaagc agagaacgcc atcgaagggg agagagagag agagagagag 360agagagagag agagagagag agagagagag aattctagaa aatctcttac ttgcttttta 420cttgtaatct ccagattagc aaagtcgaca cgtggcgcaa cggacatccc gagttgggat 480acccagtcac gtgcctcacg tgaagttggg acaaaatttt gtcccaacat ttacatttag 540cataagatag tgttactgtg atatcccgat t 571BN21：caagtgaata tcaagcatat agcttcttct ttctctctct cgtttccctt tttcccttct 60tttcaggaca tttcagatac tttctggttt tatccaatgt tgcacaaaag aaaatatgat 120tcactaacat gtcaatacgg gaaagagaac tcaatcaaat caaatcaaat caaacgatat 180cacagacaac aatcacgtat catgaattac tggtcacaca tattcttgtg atagtcaaga 240ctagatcaag aaatcatgca ttttcctttt atacatagca gagaaaacct tagacttgta 300tatatgtctg tgtgtgtgtg tgtgtgtgag agagagagag agagagagag agagagagag 360agagagagag agagagagag agaattttgc acgtaactgg caataattgg ccgtgtattc 420caaaaaactt ggaaaatgaa ggcatagcca aaagcatctt caatcttcat ctccggggtc 480gattggtaat ttacacaatc ttcttcttat gatatgttat tgtttgatca aagaaatttt 540atgttgaacg atttttcatt gctgtttata acttcaggga aattacagaa atggacgctt 600catgtcctga ttatgattat catctttacc ctttgaccag cttgcagatt ggg 653BN22，BN23，BN24：taataataaa caataataat aataataata atcacctagc actggggact cttctgggta 60gctggggctc gagctcagtg ggcaagccca ggaagccatt gaagcggtcg aaggtcacgt 120gcgacacgtg cctgacctct gtgggccagc tgatctccaa cgccgacgac gaccccgacg 180ccgacgccga cgacgacgtc gacgacacgt cgtctctctc gacgctgcat gtgactatgg 240acttcctcag agccgtcacc agcacatcca cgatcgcgaa ctgatgagag ccattgccgt 300cgtctctgcc attatcgccg ccgtggtaaa gccgagcctt gctgctaaac ggcgtcgtca 360cggggttgta caacgcctcg tcttcgccgt cttcgtacaa ttcgttctcc gactcttctt 420cttcttcttc ttcttcttcg tcggattctt gttcgtctat gaagataggg ctgggtgggg 480ccgtgttgaa ctcggcgagc ccgcaagatt ttgaccggaa aagccgagtc atggcgagag 540gtcaagatca gagaaaccaa aacagagtag tgtttgtgtg tttgatttgt tttgagagtt 600gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga gagagagaag 660agccggcctt ggaagaaaca agaaggagaa gagaaaagct tgatttttcc aagctaaaac 720gtaaggtaac ttagccagct tttcttatag tttgagtttt 760BN25：taacaactca ccactgtgat ggaccaattg actcatcgtc aacagaatga ttgatcggct 60cactttacaa cgatcggtcg gctccttgca atgaccgatt ggtccagttg gctcgtcacc 120gacatgatga cgagttggtt ctccttctca acgttagatt ggctcgcttt ctggccacca 180acataatgat taaataaatt ttattctctc tcaactcata ccaataatta cttcacaaat 240atcatttttc tacttctttt atcttttctc tctaggctct ctctttcttc cgatcttctt 300tcttttctct ctctctctct ctctctctct ctctctctct ctctctctgg aattctcgct 360ccctctctgt ttcatctctc acactctccc tctctctcat atctgctctg atcactatac 420aacggggtta gactttggag tttgaatatg ttattacgct tgatgttggc tgttctctct 480tctaagctca aaactgaaat actttttttt ttactggtat tgatagaat 529BN26：taacccaact caagctgaag aaacttattc aatggtcacc gctaaccgct tttggtccca 60aatctttggg gttgcttttt ccaataaacg ttggttacat ttctttatgt tatttgtacc 120agtcactggt ttatggatga gtgctcttgg agtagtcggt ctggctttga atctacgtgc 180ctatgacttc gtttctcaag aaatccgtgc agcggaagat cctgaatttg agactttcta 240cacaaaaaat atcctcttaa tttgttgttt gtgtgtcagt gtttgtggtg atttcagggg 300agacagagag agagagagat tgtgagaaag agaaagagag agagagagat gaaagtgaag 360gctgaattat tggaaagata gatacagaga catgtcgttt gtgggttctg agttggaatt 420ggttgagata taatcatgcc ggccaatggc aatcacggaa agtttagttt tt 472BN27：taagatgtac caaagaaatt ggggggccag agaagcacaa gagagaggct aatctggaca 60taagaacccc caacactttg gaagtggaat caatatatgc ctctagaaca aagccatcaa 120gaaatcgatc atcagatctc aaacccttga agccgaaagg agaggcattt atactcaacc 180cacgcacaca gagccaaaat gaaaattgct ttacaccaaa gagagagaga gagagggagt 240aatgattcga ggcttagtga agagtagagt tgtgaaggat atttataggg tggcctaaaa 300ccctaggttg tgaacgatgc tgagaagata tcagctgc 338BN28：acaaaacctc cccaatcaat ttcaagtttg gaggccgacg atggtgaggt ggatggctgc 60ggcggctgtg cgattttcgg catagtccga cagtttccga tgacaaacca ctgctcaaaa 120tattcctctc gtcgagagct ttccaacggt accaagtatg catcaatcgg acttaggatg 180agaaagttat gagtgttcga attttgaaga atttttgaaa aaaataattg aggagagaaa 240gagaattcga atttagagaa gaaagagaga gagggtgaca gaaatagggg gaagaagaag 300aagaaaagga aaagaaaagg ggaaaaagaa aaagaaaaag tgatgtaatg ggttggtgag 360tgtatt 366BN29：taatcatcaa tccatgtcgt tttcagcctt gccgcttctt cagtaggtct cacttgttct 60ctaattgtcg ttttcacgtc tccttcgaca acaaaaacga cacgtcgttt acatcccccg 120ctctcgcagc ctcaaaaacc cccaaaaacc ccaacaaatc tccttcggcg tttagcttct 180cagattgaga cacagagaga gagagagaga gagagagaga gagagagagg agagagagag 240aggggtgggt cagtacatgt ggcagattcg cacgtgcctc tttgtgccct tggaaaatgc 300ccaaaattag cacttggcct ctgcaaaaac tagtactttg agttgagcaa catatatgaa 360ttcacatttc cacgctaata cactatcttt cctattatct cttaaaagaa ataaaaaaaa 420tttccaaaat acgaaggaac aattttcatt gaaactacga taaatcaatg ttcctagaca 480tttgttgttg caaataccaa acataatcac tttcttcacc tcttgcatct tgaatttcct 540tt 542BN30：taagaaagac tttttttgac caaaatgatc atatcataca ttagtcaact tgattttgat 60aaaatcaagg agcttggcat ttgaagaata gttctatatg tactactttt tttttagaaa 120aaaatgtgat tgactttgat ataattaaac atttataaac cttttgaatt agcagagaaa 180agggcagttg gttattattg agctagctca atacagtttg gtcaggctga gaaaattggc 240gatataagta gatatctaat tttacctata ataagtcata ggcaaatgat gcactaaacg 300caaatttcca agatgattta agggtccaaa atccttttat acttaggtga gtttttagag 360agagtttcac ttgtgtcttt ctctctctct ctctctctct ctctctctct ctctctctct 420ctctatctct atatccattt acagctccat tttctttatg aatgaatgtt gacgcacgct 480ttcacctaac tctgctttct aatctaaaat caagattgga agagttttat gttttctttt 540tcgacttcta gagataacat tttttttggg tattgcgtat gatgattgga tataaatgtt 600gactttttaa agggcttttt ttggcaccga aatgactatg gcgaagttgt tttgatccca 660aatgagtgtt 670BN31：taaaaaatgg agacaccatt atcatctaaa attcgaagaa aattcaaaag cagtgacagg 60agttgtagac ttacacccat acgtttgccc ttgcctccag ccagaagaat gaccgaaacg 120cttttttcct ttactaaacc aaccttttca gcagaacaac aaaccaacaa acttggcttt 180gaaccaactc ttccacgtct cactgcacgc ccaacaattg caagaccatg taaagattca 240gaatctattt aggaaaacag accaaaaaaa aaaaaaaggg gactacttgc ctgggaatgg 300aagattgaaa tgcggaagtg tgtgagaaag agggatagtc ctcacatcgg aagaaaaagc 360agaaaaggtg aggctcagac ggagagtggg agtgggtatg gccatatctg ttttagtgtt 420ttcggattgg aagtggcatt cactgccact gctgactttg atacgcagag cacaacgcaa 480cacagaatga agttcgggcg gcgatggatg gcttggggtt tctcgagacg atgggcggtg 540gtcggagggg aaggtacgcc gcgattctca gcggcagagg cgacggcgtt cggcagtgat 600tttgggtggt tcaattttgg gtttctctcg gaaaaagatg gcggtgaccg actttgttgc 660cggcagttgg ataacaaaga tcgagagaga gagagagaga gagagagaga gagagtgagt 720acgctgttcc tggaagtagt agtttccttg gaggcgacag ccgaggcgat cgatggcttg 780gccggtctcg cggatccaga atccgacggt gtaggttgct ttgcccaaag tcccatctta 840gcgagctcgg agtcgtcgtc ttcagtgggg atttaggttt ctgagtcaaa cagaccaaga 900ttttgatttc gggagaagag aaatgatacc cgagaaagcg ggggattggg gtataaaacg 960acgactcacc ggtcgtggat gttttttttt gacaattacg tgattcccat tttaccaggg 1020gacgaattca tctatcctaa agattccgta ccgactcaat tcaacttg 1068BN32：taatcgcctt actagttgct gtactgatat acttgggagc gaagaaaaga aaaatgatca 60agctcaagga gaaattcttt gtagaaaatg gaggattgat gttacaacaa caacttgcta 120gtagaagacc agtcgagacg acaaaaatat tttctgagga cgaactgaga agcgccacaa 180acaattacca ccaaaagaga gttctaggag aaggaggatt tggaatagtg tataaaggaa 240tatttccaga tcaaagggtt gttgcaatta ataacacctc aaaaaagctc agaaaaaaaa 300aaaaaccaga aaccaaaatc atgtttgttt gagagagaga gagagaggga gagagagaga 360gagagagaga gagagagtcc aagtctcttt gtccttctac gccagcctct gacacgtgct 420ctcatggggt tgtcctattc tcatctcatc tcatcatgct tggccttggc ctttgccttc 480ccaatttttc ctctgtaaca ctcttctatt caaaataatc tcatattgtt gatattcaat 540caaaagaaga ggcgctattg tttatttgtt ttcatactgc aaattctacg cgtatatgta 600cgcacatgaa aatgtattag agttattatt gcgttattta taagtgaga 649BN33：attctctctc ttcaaaggta aagtttctct ctctctctct ctcctttttt ttttctgtga 60atgtatagta atggaatgaa tgtcaagaaa ccatgctcat gcaagttctg cgatgatagt 120tttggtgacg ggtttcgctt ctgttctctt agtttgtgcc gaatttagca ttttcgtagg 180catttcttag ggtcggaagg tagtgattgg agattctagt ttcggtaatg gcggatcttt 240aggttctact ctctgattgt ggtttttttc tgtatggttg tgttcgttgc gtgttggatc 300tgtgggaaat tttggatttt ttttgg 326。