| 摘要 |
FIELD: chemistry of peptides, chemical technology. ^ SUBSTANCE: invention relates to a method for purification of nona- or decapeptide representing antagonist of luteinizing hormone releasing factor (LHRH) from residual organic solvent. Method involves dissolving nona- or decapeptide in a water-containing solvent and at least one (C1-C3)-alcohol followed by precipitation in a solvent under condition with intensive stirring wherein this solvent represents carboxylic acid alkyl ester comprising from 3 to 6 carbon atoms and one or some nonpolar compounds chosen from hexane, heptane, octane, cyclohexane and methylcyclohexane and, possibly, up to 5% of acetic or propionic acid. Then precipitate of nona- or decapeptide is washed out with a mixture of (C3-C5)-esters and dried. The water content in water-containing solvent used and at least one alcohol is less 8% (vol./vol.) and in the volume ratio of solvents mixture used for dissolving and solvents mixture used for precipitation is 1:10 or above. ^ EFFECT: improved purifying method. ^ 8 cl, 1 tbl, 6 ex |
