发明名称 |
Recombinant calf-chymosin and a process for producing the same |
摘要 |
The present invention relates to a recombinant calf-Chymosin protein as set forth in SEQ ID No. 1; a recombinant calf-Chymosin gene as set forth in SEQ ID No. 2 encoding the protein comprising amino acid sequence of SEQ ID NO. 1; an E. coli comprising the recombinant chymosin gene of SEQ ID No. 2; an expression vector pET21b comprising recombinant calf-chymosin gene as set forth in SEQ ID No. 2; and lastly a method for producing recombinant calf-chymosin protein as set forth in SEQ ID No. 1 which comprises steps of isolating calf-chymosin gene, cloning the same in bacterial expression vector pET21b, transforming said cloned vector into cells of E. coli, fermenting said E. coli to produce pro-chymosin, converting said pro-chymosin to chymosin and subsequently recovering the recombinant calf-chymosin.
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申请公布号 |
US2007166785(A1) |
申请公布日期 |
2007.07.19 |
申请号 |
US20040599391 |
申请日期 |
2004.03.30 |
申请人 |
MULE VEAKATA MADHUSUDHAN R;MYTHILI PADALA K;GOPALAKRISHNA KARANAM;RAMANA YAMALA;REDDY DONTHI R B |
发明人 |
MULE VEAKATA MADHUSUDHAN R.;MYTHILI PADALA K.;GOPALAKRISHNA KARANAM;RAMANA YAMALA;REDDY DONTHI R.B. |
分类号 |
C12P21/06;C07H21/04;C12N1/21;C12N9/64;C12N15/74 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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