发明名称 |
Self-rearranging DNA vectors |
摘要 |
Disclosed are replicatable viral DNA vectors encoding a site-specific DNA-altering enzyme and a DNA target recognized by the enzyme, the enzyme selectively converting, in a cell expressing the enzyme, the DNA vector to a rearranged form. The invention further relates to methods for assembling recombinant adenoviral DNAs. These methods include the steps of: (a) providing a first linearized DNA vector including a restriction site and a cos site and a second linearized DNA vector including the restriction site, an adenoviral nucleic acid molecule, and a cos site; and (b) ligating the first and second linearized DNA vectors, the ligation assembling a recombinant adenoviral DNA.
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申请公布号 |
US2007166286(A1) |
申请公布日期 |
2007.07.19 |
申请号 |
US20070717267 |
申请日期 |
2007.03.13 |
申请人 |
SEED BRIAN;FREEMAN MASON W;KOVTUN ALEXANDER;MURAKAWA MASAHIRO;PARK EUN-CHUNG;WANG XINZHONG |
发明人 |
SEED BRIAN;FREEMAN MASON W.;KOVTUN ALEXANDER;MURAKAWA MASAHIRO;PARK EUN-CHUNG;WANG XINZHONG |
分类号 |
A61K48/00;C12N15/09;A61K35/76;A61P31/22;A61P35/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/63;C12N15/861 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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